Tri-iodothyronine ELISA Kit (Sheep)

Mean Intra-Assay Precision %CV Mean Inter-Assay Precision %CV Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean (ng/mL 1.93 1.924 1.974 2.002 1.955 1.943 SD 0.018 0.022 0.02 0.027 0.035 0.036 CV% 1.997 2.449 2.307 3.131 4.001 3.998 Mean Intra-Assay Precision %CV Mean Inter-Assay Precision %CV Mean Intra-Assay Precision %CV Mean Inter-Assay Precision %CV Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 n 20 20 20 20 20 20 Mean (ng/mL 1.93 1.924 1.974 2.002 1.955 1.943 Mean (ng/mL Mean (ng/mL 1.93 1.924 1.974 2.002 1.955 1.943 SD 0.018 0.022 0.02 0.027 0.035 0.036 SD 0.018 0.022 0.02 0.027 0.035 0.036 CV% 1.997 2.449 2.307 3.131 4.001 3.998 CV% 1.997 2.449 2.307 3.131 4.001 3.998

The L-triiodothyronine (T3, liothyronine) thyroid hormone is normally synthesized and secreted by the thyroid gland in much smaller quantities than L-tetraiodothyronine (T4, levothyroxine, L-thyroxine) . Most T3 is derived from peripheral monodeiodination of T4 at the 5 position of the outer ring of the iodothyronine nucleus. The hormone finally delivered and used by the tissues is mainly T3.

Aviva Systems Biology Tri-iodothyronine ELISA Kit (Sheep) (OKCA00334) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Sheep Tri-iodothyronine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Tri-iodothyronine and incubated. The Tri-iodothyronine found in the sample or standards competes with the biotinylated Tri-iodothyronine for limited binding sites on the immobilized anti-Sheep Tri-iodothyronine antibody. Excess unbound biotinylated Tri-iodothyronine and sample or standard Tri-iodothyronine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Tri-iodothyronine captured in the well and inversely proportional to the amount of Tri-iodothyronine which was contained in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample 1 Sample Type Average Recovery Range Serum (n = 5) 0.96 85-102% Sample 1 Sample 1 Sample Type Average Recovery Range Sample Type Average Recovery Range Serum (n = 5) 0.96 85-102% Serum (n = 5) 0.96 85-102% Sample 2 Sample Type Average Recovery Range EDTA plasma (n = 4) 0.96 90-100% Sample 2 Sample 2 Sample Type Average Recovery Range Sample Type Average Recovery Range EDTA plasma (n = 4) 0.96 90-100% EDTA plasma (n = 4) 0.96 90-100%