ADIPOQ ELISA Kit (Hamster)

Aviva Systems Biology ADIPOQ ELISA Kit (Hamster) (OKCA00304) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for hamster ADIPOQ has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for hamster ADIPOQ is added, incubated and then followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP producing a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample hamster ADIPOQ captured in well.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample 1 Sample Type Average Recovery Range Serum (n=5) 88% 84-92% Sample 1 Sample 1 Sample Type Average Recovery Range Sample Type Average Recovery Range Serum (n=5) 88% 84-92% Serum (n=5) 88% 84-92% Sample 2 Sample Type Average Recovery Range EDTA Plasma (n=4) 95% 90-100% Sample 2 Sample 2 Sample Type Average Recovery Range Sample Type Average Recovery Range EDTA Plasma (n=4) 95% 90-100% EDTA Plasma (n=4) 95% 90-100%