Thyroxine ELISA Kit (Goat)

Mean Intra-Assay Precision %CV Mean Inter-Assay Precision %CV Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean (ng/mL 80.668 80.588 80.528 80.221 80.786 80.81 SD 0.054 0.076 0.065 0.081 0.054 0.046 CV% 5.574 7.849 6.702 8.372 5.584 4.794 Mean Intra-Assay Precision %CV Mean Inter-Assay Precision %CV Mean Intra-Assay Precision %CV Mean Inter-Assay Precision %CV Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 n 20 20 20 20 20 20 Mean (ng/mL 80.668 80.588 80.528 80.221 80.786 80.81 Mean (ng/mL Mean (ng/mL 80.668 80.588 80.528 80.221 80.786 80.81 SD 0.054 0.076 0.065 0.081 0.054 0.046 SD 0.054 0.076 0.065 0.081 0.054 0.046 CV% 5.574 7.849 6.702 8.372 5.584 4.794 CV% 5.574 7.849 6.702 8.372 5.584 4.794

Aviva Systems Biology Thyroxine ELISA Kit (Goat) (OKCA00273) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Goat Thyroxine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Thyroxine and incubated. The Thyroxine found in the sample or standards competes with the biotinylated Thyroxine for limited binding sites on the immobilized anti-Goat Thyroxine antibody. Excess unbound biotinylated Thyroxine and sample or standard Thyroxine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Thyroxine captured in the well and inversely proportional to the amount of Thyroxine which was contained in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample 1 Sample Type Average Recovery Range Serum (n = 5) 0.97 92-110% Sample 1 Sample 1 Sample Type Average Recovery Range Sample Type Average Recovery Range Serum (n = 5) 0.97 92-110% Serum (n = 5) 0.97 92-110% Sample 2 Sample Type Average Recovery Range EDTA plasma (n = 4) 0.95 90-105% Sample 2 Sample 2 Sample Type Average Recovery Range Sample Type Average Recovery Range EDTA plasma (n = 4) 0.95 90-105% EDTA plasma (n = 4) 0.95 90-105%