Goat IgG ELISA Kit (Goat)

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean (ug/mL) 2.377 2.350 2.442 2.341 2.405 2.288 SD 0.021 0.026 0.027 0.036 0.041 0.044 CV% 3.328 4.101 4.327 5.669 6.529 6.864 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 n 20 20 20 20 20 20 Mean (ug/mL) 2.377 2.350 2.442 2.341 2.405 2.288 Mean (ug/mL) 2.377 2.350 2.442 2.341 2.405 2.288 SD 0.021 0.026 0.027 0.036 0.041 0.044 SD 0.021 0.026 0.027 0.036 0.041 0.044 CV% 3.328 4.101 4.327 5.669 6.529 6.864 CV% 3.328 4.101 4.327 5.669 6.529 6.864

Aviva Systems Biology Goat IgG ELISA Kit (Goat) (OKCA00272) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with Goat IgG and blocked. Standards or samples are added to the plate wells along with a horseradish peroxidase (HRP) conjugated antibody specific to Goat IgG and incubated. Goat IgG immobilized in the wells competes with the liquid phase Goat IgG contained in the samples or standards for binding with the HRP-Conjugated Goat IgG Antibody. Wells are washed removing unbound Goat IgG, unbound HRP-Conjugated Goat IgG Antibody or Goat IgG /HRP-Conjugated Goat IgG Antibody complex. HRP-Conjugated Goat IgG Antibody bound to Goat IgG immobilized in the well remains. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of HRP-Conjugated Goat IgG Antibody captured in the well and inversely proportional to the amount of Goat IgG which was contained in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol