Lysozyme ELISA Kit (Chicken)

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean (ng/mL) 249.61 247.756 250.857 248.99 246.53 254.012 SD 0.026 0.029 0.031 0.039 0.041 0.046 CV% 3.621 4.022 4.33 5.424 5.671 6.471 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 n 20 20 20 20 20 20 Mean (ng/mL) 249.61 247.756 250.857 248.99 246.53 254.012 Mean (ng/mL) 249.61 247.756 250.857 248.99 246.53 254.012 SD 0.026 0.029 0.031 0.039 0.041 0.046 SD 0.026 0.029 0.031 0.039 0.041 0.046 CV% 3.621 4.022 4.33 5.424 5.671 6.471 CV% 3.621 4.022 4.33 5.424 5.671 6.471

Aviva Systems Biology Lysozyme ELISA Kit (Chicken) (OKCA00263) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Chicken Lysozyme antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Lysozyme and incubated. The Lysozyme found in the sample or standards competes with the biotinylated Lysozyme for limited binding sites on the immobilized anti-Chicken Lysozyme antibody. Excess unbound biotinylated Lysozyme and sample or standard Lysozyme is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Lysozyme captured in the well and inversely proportional to the amount of Lysozyme which was contained in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol