Free Tri-iodothyronine ELISA Kit (Canine)

Mean Intra-Assay Precision %CV Mean Inter-Assay Precision %CV Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean (pmol/L) 5.375 5.465 5.423 5.387 5.524 5.518 SD 0.039 0.041 0.037 0.046 0.045 0.044 CV% 2.843 2.964 2.716 3.3 3.302 3.222 Mean Intra-Assay Precision %CV Mean Inter-Assay Precision %CV Mean Intra-Assay Precision %CV Mean Inter-Assay Precision %CV Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 n 20 20 20 20 20 20 Mean (pmol/L) 5.375 5.465 5.423 5.387 5.524 5.518 Mean (pmol/L) 5.375 5.465 5.423 5.387 5.524 5.518 SD 0.039 0.041 0.037 0.046 0.045 0.044 SD 0.039 0.041 0.037 0.046 0.045 0.044 CV% 2.843 2.964 2.716 3.3 3.302 3.222 CV% 2.843 2.964 2.716 3.3 3.302 3.222

3,3',5-Triiodo-L-thyronine, 3,3',5-Triiodothyronine, 3,5,3'-Triiodothyronine, 4- (4-Hydroxy-3-iodophenoxy) -3,5-diiodophenylalanine, Alanine, 3-[4- (4-hydroxy-3-iodophenoxy) -3,5-diiodophenyl]-, L-Cyronine, L-3,3',5-Triiodothyronine, othyronin, Liothyronin, l

An iodothyronine compound having iodo substituents at the 3-, 3'- and 5-positions. Although some is produced in the thyroid, most of the 3,3',5-triiodo-L-thyronine in the body is generated by mono-deiodination of L-thyroxine in the peripheral tissues. Its metabolic activity is about 3 to 5 times that of L-thyroxine. The sodium salt is used in the treatment of hypothyroidism.

Aviva Systems Biology Free Tri-iodothyronine ELISA Kit (Canine) (OKCA00253) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Canine Free Tri-iodothyronine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Free Tri-iodothyronine and incubated. The Free Tri-iodothyronine found in the sample or standards competes with the biotinylated Free Tri-iodothyronine for limited binding sites on the immobilized anti-Canine Free Tri-iodothyronine antibody. Excess unbound biotinylated Free Tri-iodothyronine and sample or standard Free Tri-iodothyronine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Free Tri-iodothyronine captured in the well and inversely proportional to the amount of Free Tri-iodothyronine which was contained in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

This assay has high sensitivity and excellent specificity for detection of canine Free-T3. No significant cross-reactivity or interference between canine Free-T3 and analogues was observed.