CCL5 ELISA Kit (Canine)

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean (ng/ml) 1.234 1.208 1.253 1.225 1.227 1.202 SD 0.026 0.022 0.028 0.046 0.049 0.051 CV% 2.377 2.035 2.539 4.224 4.495 4.731 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 n 20 20 20 20 20 20 Mean (ng/ml) 1.234 1.208 1.253 1.225 1.227 1.202 Mean (ng/ml) 1.234 1.208 1.253 1.225 1.227 1.202 SD 0.026 0.022 0.028 0.046 0.049 0.051 SD 0.026 0.022 0.028 0.046 0.049 0.051 CV% 2.377 2.035 2.539 4.224 4.495 4.731 CV% 2.377 2.035 2.539 4.224 4.495 4.731

This gene is one of several CC cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor CCR5 and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor.

Aviva Systems Biology CCL5 ELISA Kit (Canine) (OKCA00238) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Canine CCL5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Canine CCL5 is added, incubated and then followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP producing a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Canine CCL5 captured in well.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample 1 Sample Type Average Recovery Range Serum (n=5) 104% 96-108% Sample 1 Sample 1 Sample Type Average Recovery Range Sample Type Average Recovery Range Serum (n=5) 104% 96-108% Serum (n=5) 104% 96-108% Sample 2 Sample Type Average Recovery Range EDTA Plasma (n=4) 99% 91-104% Sample 2 Sample 2 Sample Type Average Recovery Range Sample Type Average Recovery Range EDTA Plasma (n=4) 99% 91-104% EDTA Plasma (n=4) 99% 91-104%

This assay has high sensitivity and excellent specificity for detection of dog CCL5. No significant cross-reactivity or interference between dog CCL5 and analogues was observed.