AMBP ELISA Kit (Rat)

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean (ng/ml) 12.666 12.603 12.294 12.839 12.927 12.888 SD 0.033 0.053 0.025 0.034 0.066 0.073 CV% 3.946 6.38 2.909 4.104 8.101 8.954 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 n 20 20 20 20 20 20 Mean (ng/ml) 12.666 12.603 12.294 12.839 12.927 12.888 Mean (ng/ml) 12.666 12.603 12.294 12.839 12.927 12.888 SD 0.033 0.053 0.025 0.034 0.066 0.073 SD 0.033 0.053 0.025 0.034 0.066 0.073 CV% 3.946 6.38 2.909 4.104 8.101 8.954 CV% 3.946 6.38 2.909 4.104 8.101 8.954

This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes.

Aviva Systems Biology AMBP ELISA Kit (Rat) (OKCA00171) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with Rat AMBP and blocked. Standards or samples are added to the plate wells along with a horseradish peroxidase (HRP) conjugated antibody specific to AMBP and incubated. AMBP immobilized in the wells competes with the liquid phase AMBP contained in the samples or standards for binding with the HRP-Conjugated AMBP Antibody. Wells are washed removing unbound AMBP, unbound HRP-Conjugated AMBP Antibody or AMBP/HRP-Conjugated AMBP Antibody complex. HRP-Conjugated AMBP Antibody bound to AMBP immobilized in the well remains. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of HRP-Conjugated AMBP Antibody captured in the well and inversely proportional to the amount of AMBP which was contained in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample 1 Sample Type Average Recovery Range Serum (n=5) 91% 87-95% Sample 1 Sample 1 Sample Type Average Recovery Range Sample Type Average Recovery Range Serum (n=5) 91% 87-95% Serum (n=5) 91% 87-95% Sample 2 Sample Type Average Recovery Range EDTA Plasma (n=4) 99% 99-104% Sample 2 Sample 2 Sample Type Average Recovery Range Sample Type Average Recovery Range EDTA Plasma (n=4) 99% 99-104% EDTA Plasma (n=4) 99% 99-104%