Luteinizing Hormone ELISA Kit (Mouse)

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean (mIU/mL) 20.348 18.906 19.157 20.022 18.69 19.733 SD 0.041 0.038 0.04 0.057 0.063 0.063 CV% 6.077 5.984 6.231 8.557 10.011 9.574 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 n 20 20 20 20 20 20 Mean (mIU/mL) 20.348 18.906 19.157 20.022 18.69 19.733 Mean (mIU/mL) 20.348 18.906 19.157 20.022 18.69 19.733 SD 0.041 0.038 0.04 0.057 0.063 0.063 SD 0.041 0.038 0.04 0.057 0.063 0.063 CV% 6.077 5.984 6.231 8.557 10.011 9.574 CV% 6.077 5.984 6.231 8.557 10.011 9.574

Aviva Systems Biology Luteinizing Hormone ELISA Kit (Mouse) (OKCA00156) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A mouse monoclonal antibody specific for Mouse Luteinizing Hormone has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells and incubated. After washing, a biotinylated detector antibody specific for Mouse Luteinizing Hormone is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Mouse Luteinizing Hormone captured in well.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol