AMBP ELISA Kit (Mouse)

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean (ng/ml) 12.57 12.507 12.004 12.739 12.827 12.789 SD 0.032 0.052 0.024 0.033 0.065 0.072 CV% 3.927 6.361 2.86 4.085 8.082 8.935 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 n 20 20 20 20 20 20 Mean (ng/ml) 12.57 12.507 12.004 12.739 12.827 12.789 Mean (ng/ml) 12.57 12.507 12.004 12.739 12.827 12.789 SD 0.032 0.052 0.024 0.033 0.065 0.072 SD 0.032 0.052 0.024 0.033 0.065 0.072 CV% 3.927 6.361 2.86 4.085 8.082 8.935 CV% 3.927 6.361 2.86 4.085 8.082 8.935

This gene encodes a fusion protein that undergoes proteolytic processing to generate two mature proteins: alpha-1-microglobulin (A1m) is a heme-binding plasma glycoprotein of the lipocalin superfamily of proteins that bind to hydrophobic molecules, whereas bikunin belongs to the superfamily of Kunitz-type protease inhibitors. The transgenic mice specifically lacking bikunin, but not A1m, exhibit female infertility and an increased sensitivity to lung metastasis.

Aviva Systems Biology AMBP ELISA Kit (Mouse) (OKCA00132) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with Mouse AMBP and blocked. Standards or samples are added to the plate wells along with a horseradish peroxidase (HRP) conjugated antibody specific to AMBP and incubated. AMBP immobilized in the wells competes with the liquid phase AMBP contained in the samples or standards for binding with the HRP-Conjugated AMBP Antibody. Wells are washed removing unbound AMBP, unbound HRP-Conjugated AMBP Antibody or AMBP/HRP-Conjugated AMBP Antibody complex. HRP-Conjugated AMBP Antibody bound to AMBP immobilized in the well remains. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of HRP-Conjugated

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample 1 Sample Type Average Recovery Range Serum (n=5) 90% 87-93% Sample 1 Sample 1 Sample Type Average Recovery Range Sample Type Average Recovery Range Serum (n=5) 90% 87-93% Serum (n=5) 90% 87-93% Sample 2 Sample Type Average Recovery Range EDTA Plasma (n=4) 97% 92-102% Sample 2 Sample 2 Sample Type Average Recovery Range Sample Type Average Recovery Range EDTA Plasma (n=4) 97% 92-102% EDTA Plasma (n=4) 97% 92-102%