CA50 ELISA Kit (Human)

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean (U/mL) 39.516 41.384 42.702 41.107 38.137 40.622 SD 0.047 0.054 0.053 0.061 0.059 0.066 CV% 7.321 8.019 7.594 9.157 9.387 9.957 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 n 20 20 20 20 20 20 Mean (U/mL) 39.516 41.384 42.702 41.107 38.137 40.622 Mean (U/mL) 39.516 41.384 42.702 41.107 38.137 40.622 SD 0.047 0.054 0.053 0.061 0.059 0.066 SD 0.047 0.054 0.053 0.061 0.059 0.066 CV% 7.321 8.019 7.594 9.157 9.387 9.957 CV% 7.321 8.019 7.594 9.157 9.387 9.957

Aviva Systems Biology CA50 ELISA Kit (Human) (OKCA00106) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A mouse monoclonal antibody specific for human CA50 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells and incubated. After washing, a biotinylated detector antibody specific for human CA50 is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample human CA50 captured in well.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol