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Metallothionein ELISA Kit (Human)

Product Specifications

Reproducibility

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean (ng/ml) 50.469 52.058 50.8 49.567 51.384 48.838 SD 0.036 0.028 0.027 0.08 0.088 0.057 CV% 3.367 2.574 2517 7.561 8.146 5.436 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 n 20 20 20 20 20 20 Mean (ng/ml) 50.469 52.058 50.8 49.567 51.384 48.838 Mean (ng/ml) 50.469 52.058 50.8 49.567 51.384 48.838 SD 0.036 0.028 0.027 0.08 0.088 0.057 SD 0.036 0.028 0.027 0.08 0.088 0.057 CV% 3.367 2.574 2517 7.561 8.146 5.436 CV% 3.367 2.574 2517 7.561 8.146 5.436

CAS Number

7732-18-5

Gene Name

Metallothionein 1A

Gene Aliases

MT1, MTC, MT1S, MT-1A, MT-IA

Gene ID

4489

Accession Number

NP_005937

Reactivity

Human

Type

ELISA Kit

Assay Principle

Aviva Systems Biology Metallothionein ELISA Kit (Human) (OKCA00004) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for human Metallothionein has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for human Metallothionein is added, incubated and then followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP producing a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample human Metallothionein captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Detection Range

6.25 pg/ml - 400 pg/ml

Sensitivity

< 0.813 pg/ml

Reconstitution

Store as indicated in product manual.

Molecular Weight

6 kDa

Protein Length

61

NCBI Gene Symbol

Metallothionein

Protein Name

Metallothionein-1A

Gene Name URL

Metallothionein

Nucleotide Accession Number

NM_005946

Frequently Asked Questions

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