Metallothionein ELISA Kit (Human)
Product Specifications
Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean (ng/ml) 50.469 52.058 50.8 49.567 51.384 48.838 SD 0.036 0.028 0.027 0.08 0.088 0.057 CV% 3.367 2.574 2517 7.561 8.146 5.436 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 n 20 20 20 20 20 20 Mean (ng/ml) 50.469 52.058 50.8 49.567 51.384 48.838 Mean (ng/ml) 50.469 52.058 50.8 49.567 51.384 48.838 SD 0.036 0.028 0.027 0.08 0.088 0.057 SD 0.036 0.028 0.027 0.08 0.088 0.057 CV% 3.367 2.574 2517 7.561 8.146 5.436 CV% 3.367 2.574 2517 7.561 8.146 5.436
CAS Number
7732-18-5
Gene Name
Metallothionein 1A
Gene Aliases
MT1, MTC, MT1S, MT-1A, MT-IA
Gene ID
4489
Accession Number
NP_005937
Reactivity
Human
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Metallothionein ELISA Kit (Human) (OKCA00004) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for human Metallothionein has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for human Metallothionein is added, incubated and then followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP producing a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample human Metallothionein captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Detection Range
6.25 pg/ml - 400 pg/ml
Sensitivity
< 0.813 pg/ml
Reconstitution
Store as indicated in product manual.
Molecular Weight
6 kDa
Protein Length
61
NCBI Gene Symbol
Metallothionein
Protein Name
Metallothionein-1A
Gene Name URL
Metallothionein
Nucleotide Accession Number
NM_005946
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog