CD163 ELISA Kit (Human)

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 3520 11456 44674 3368 11294 48653 Standard deviation 144.32 526.97 303195.343 195.34 632.46 3357.05 CV (%) 4.1 4.6 6.8 5.8 5.6 6.9 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 3520 11456 44674 3368 11294 48653 Mean (pg/ml) 3520 11456 44674 3368 11294 48653 Standard deviation 144.32 526.97 303195.343 195.34 632.46 3357.05 Standard deviation 144.32 526.97 303195.343 195.34 632.46 3357.05 CV (%) 4.1 4.6 6.8 5.8 5.6 6.9 CV (%) 4.1 4.6 6.8 5.8 5.6 6.9

CD163 (Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich (SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc.

The Aviva Quick Picokineâ„¢ Human CD163 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CD163 in cell culture supernatants, serum and plasma (heparin) . It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit contains recombinant Human CD163 with immunogen: Expression system for standard: NS0; Immunogen sequence: G41-S1045. To measure Human CD163, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product is linearly proportional to Human CD163 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CD163 in the sample. For more information on assay principle, protocols, and troubleshooting tips, see Aviva's ELISA Resource Center at https://www.Avivabio.com/elisa-technical-resource-center.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol