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Omd ELISA Kit (Mouse)

Product Specifications

Reproducibility

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 279 1140 4460 308 1190 4553 Standard deviation 13.39 49.02 182.86 20.33 69.02 346.03 CV (%) 4.8 4.3 4.1 6.6 5.8 7.6 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 279 1140 4460 308 1190 4553 Mean (pg/ml) 279 1140 4460 308 1190 4553 Standard deviation 13.39 49.02 182.86 20.33 69.02 346.03 Standard deviation 13.39 49.02 182.86 20.33 69.02 346.03 CV (%) 4.8 4.3 4.1 6.6 5.8 7.6 CV (%) 4.8 4.3 4.1 6.6 5.8 7.6

CAS Number

7732-18-5

Gene Name

Osteomodulin

Gene Aliases

Osteomodulin; Keratan sulfate proteoglycan osteomodulin; KSPG osteomodulin; Osteoadherin; OSAD; Omd

Gene ID

27047

Accession Number

NP_036180.1

Reactivity

Mouse

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q21-I423

Target

Osteomodulin (also called osteoadherin or osteoadherin proteoglycan) is a protein that in humans is encoded by the OMD gene. This gene is mapped to 13; 13 A5. Osteoadherin is expressed by fetal and adult osteoblasts but is not detectable in cartilage or tendon. In dental tissue, OSAD is expressed by odontoblasts and ameloblasts and is involved in the mineralization of bone and teeth. OSAD promotes the adhesion of osteoblasts and odontoblasts to the surrounding matrix, an interaction that is mediated by Integrin alpha V beta 3.

Type

ELISA Kit

Applications

ELISA

Assay Principle

Aviva's mouse Osteoadherin ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Osteoadherin has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: Q21-I423) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Osteoadherin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse Osteoadherin amount of sample captured in plate.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Cell culture supernates, serum and plasma (heparin, EDTA)

Detection Range

156 pg/ml - 10,000 pg/ml

Sensitivity

<50 pg/ml

Reconstitution

Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Specificity

Natural and recombinant mouse Osteoadherin

NCBI Gene Symbol

OMD

Protein Name

Osteomodulin

Gene Name URL

OMD

Nucleotide Accession Number

NM_012050.2

Frequently Asked Questions

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