TREML2 ELISA Kit (Human)
Product Specifications
Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 94 472 1928 111 507 1976 Standard deviation 6.30 24.54 109.90 6.99 27.38 81.02 CV (%) 6.7 5.2 5.7 6.3 5.4 4.1 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 94 472 1928 111 507 1976 Mean (pg/ml) 94 472 1928 111 507 1976 Standard deviation 6.30 24.54 109.90 6.99 27.38 81.02 Standard deviation 6.30 24.54 109.90 6.99 27.38 81.02 CV (%) 6.7 5.2 5.7 6.3 5.4 4.1 CV (%) 6.7 5.2 5.7 6.3 5.4 4.1
CAS Number
7732-18-5
Gene Name
Triggering receptor expressed on myeloid cells like 2
Gene Aliases
Trem-like transcript 2 protein; TLT-2; Triggering receptor expressed on myeloid cells-like protein 2; TREML2; C6orf76, TLT2; UNQ6268/PRO20473
Gene ID
79865
Accession Number
NP_079083.2
Reactivity
Human
Immunogen
Expression system for standard: E.coli; Immunogen sequence: G19-S268
Target
TREML2 is located in a gene cluster on chromosome 6 with the single Ig variable (IgV) domain activating receptors TREM1 (MIM 605085) and TREM2 (MIM 605086), but it has distinct structural and functional properties. This gene is mapped to 6p21.1.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's human TREML2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TREML2 has been precoated onto 96-well plates. Standards (Expression system for standard: E.coli, Immunogen sequence: G19-S268) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TREML2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substmousee TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Fibronectin amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Cell culture supernates, serum and plasma (heparin, EDTA)
Detection Range
62.5 pg/ml - 4,000 pg/ml
Sensitivity
<50 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant human TREmL2
NCBI Gene Symbol
TREML2
Protein Name
Trem-like transcript 2 protein
Gene Name URL
TREML2
Nucleotide Accession Number
NM_024807.3
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog