C1ra ELISA Kit (Mouse)

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1004 3663 23406 1174 4272 24979 Standard deviation 47.19 197.80 1451.17 79.83 196.51 1448.78 CV (%) 4.7 5.4 6.2 6.8 4.6 5.8 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1004 3663 23406 1174 4272 24979 Mean (pg/ml) 1004 3663 23406 1174 4272 24979 Standard deviation 47.19 197.80 1451.17 79.83 196.51 1448.78 Standard deviation 47.19 197.80 1451.17 79.83 196.51 1448.78 CV (%) 4.7 5.4 6.2 6.8 4.6 5.8 CV (%) 4.7 5.4 6.2 6.8 4.6 5.8

Complement C1r subcomponent is a protein involved in the complement system of the innate immune system. In humans, C1r is encoded by the C1R gene. It is mapped to 6; 6 F2 and 6 F2; 6. C1r has been shown to interact with C1s. C1r cleaves C1s to form the active form of C1s.

Aviva's mouse C1R ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for C1R has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: S18-D705) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for C1R is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Fibronectin amount of sample captured in plate.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol