What is the catalog number for GPC5 ELISA Kit (Human)?

The catalog number for GPC5 ELISA Kit (Human) is OKBB01206-96W.

What sizes are available for GPC5 ELISA Kit (Human)?

GPC5 ELISA Kit (Human) is available in the following size(s): 96 Well With Removable Strips.

Is GPC5 ELISA Kit (Human) in stock?

GPC5 ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

Can I return this product if I ordered the wrong one?

As a consumer, you have a 14-day right of withdrawal from receipt under EU consumer protection law, submitted via our withdrawal/cancellation request form. Note this applies to unused, undamaged products, and doesn’t override the expiry/storage conditions on performance-based claims.

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.

Can I request additional validation data, such as western blot images, for this product?

Additional data may be available on request. Contact our technical support team with the catalogue number and we’ll check what supporting documentation the supplier can provide.

GPC5 ELISA Kit (Human)

Product Specifications

Reproducibility

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 60 226 938 62 243 967 Standard deviation 2.58 11.07 50.7 4.15 16.77 72.5 CV (%) 4.3 4.9 5.4 6.7 6.9 7.5 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 60 226 938 62 243 967 Mean (pg/ml) 60 226 938 62 243 967 Standard deviation 2.58 11.07 50.7 4.15 16.77 72.5 Standard deviation 2.58 11.07 50.7 4.15 16.77 72.5 CV (%) 4.3 4.9 5.4 6.7 6.9 7.5 CV (%) 4.3 4.9 5.4 6.7 6.9 7.5

CAS Number

7732-18-5

Gene Name

Glypican 5

Gene Aliases

Glypican-5; Secreted glypican-5; GPC5

Gene ID

2262

Accession Number

NP_004457.1

Reactivity

Human

Immunogen

Expression system for standard: NS0; Immunogen sequence: E25-T554

Target

Glypican-5 is a protein that in humans is encoded by the GPC5 gene. This gene is mapped to 13q31.3. Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

Type

ELISA Kit

Applications

ELISA

Assay Principle

Aviva's human Glypican-5/GPC5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Glypican-5/GPC5 has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: E25-T554) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Glypican-5/GPC5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Fibronectin amount of sample captured in plate.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol