GREM2 ELISA Kit (Mouse)

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 95 279 843 99 268 803 Standard deviation 5.6 13.67 34.56 6.03 18.22 35.33 CV (%) 5.9 4.9 4.1 6.1 6.8 4.4 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 95 279 843 99 268 803 Mean (pg/ml) 95 279 843 99 268 803 Standard deviation 5.6 13.67 34.56 6.03 18.22 35.33 Standard deviation 5.6 13.67 34.56 6.03 18.22 35.33 CV (%) 5.9 4.9 4.1 6.1 6.8 4.4 CV (%) 5.9 4.9 4.1 6.1 6.8 4.4

GREM2, also known as PRDC, is one of several BMP antagonists that regulate the functions of BMPs during embryonic development and tissue differentiation. BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its direct binding to BMP proteins. The PRDC gene is mapped to chromosome 1q43.

Aviva's mouse GREM2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for GREM2 has been precoated onto 96-well plates. Standards (Expression system for standard: E.coli, Immunogen sequence: R22-Q168) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GREM2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse GREM2 amount of sample captured in plate.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol