What is the catalog number for RSPO1 ELISA Kit (Human)?

The catalog number for RSPO1 ELISA Kit (Human) is OKBB01050-96W.

What sizes are available for RSPO1 ELISA Kit (Human)?

RSPO1 ELISA Kit (Human) is available in the following size(s): 96 Well With Removable Strips.

Is RSPO1 ELISA Kit (Human) in stock?

RSPO1 ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What expression system was used for this recombinant protein?

The expression system, such as E. coli, HEK293, or insect cells, is listed on the datasheet under production details, which can affect post-translational modifications relevant to your application.

Can I return this product if I ordered the wrong one?

As a consumer, you have a 14-day right of withdrawal from receipt under EU consumer protection law, submitted via our withdrawal/cancellation request form. Note this applies to unused, undamaged products, and doesn’t override the expiry/storage conditions on performance-based claims.

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.

RSPO1 ELISA Kit (Human)

Product Specifications

Reproducibility

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 102 315 845 98 304 805 Standard deviation 5.2 16.06 42.25 6.46 16.41 45.08 CV (%) 5.1 5.1 5 6.6 5.4 5.6 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 102 315 845 98 304 805 Mean (pg/ml) 102 315 845 98 304 805 Standard deviation 5.2 16.06 42.25 6.46 16.41 45.08 Standard deviation 5.2 16.06 42.25 6.46 16.41 45.08 CV (%) 5.1 5.1 5 6.6 5.4 5.6 CV (%) 5.1 5.1 5 6.6 5.4 5.6

CAS Number

7732-18-5

Gene Name

R-spondin 1

Gene Aliases

CRISTIN3, hRspo1, R spondin1, R-spondin-1, RSPO, Rspo1, Q2MKA7

Gene ID

284654

Accession Number

NP_001033722.1

Reactivity

Human

Immunogen

Expression system for standard: NS0; Immunogen sequence: S21-A263

Target

R-spondin-1 is a secreted protein that in humans is encoded by the Rspo1 gene. It is mapped to chromosome 1p34. This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy- induced adverse effects. Alternative splicing results in multiple transcript variants.

Type

ELISA Kit

Applications

ELISA

Assay Principle

Aviva's human R-Spondin-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for R-Spondin-1 has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: S21-A263) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for R-Spondin-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human R-Spondin-1 amount of sample captured in plate.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol