ORM1 ELISA Kit (Human)

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2696 12095 38792 2753 12889 38542 Standard deviation 164.45 931.31 1784.43 192.71 1198.67 2235.43 CV (%) 6.1 7.7 4.6 7 9.3 5.8 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 2696 12095 38792 2753 12889 38542 Mean (pg/ml) 2696 12095 38792 2753 12889 38542 Standard deviation 164.45 931.31 1784.43 192.71 1198.67 2235.43 Standard deviation 164.45 931.31 1784.43 192.71 1198.67 2235.43 CV (%) 6.1 7.7 4.6 7 9.3 5.8 CV (%) 6.1 7.7 4.6 7 9.3 5.8

Alpha-1-acid glycoprotein, or orosomucoid, is a relatively abundant plasma protein synthesized in the liver. It has a molecular weith of 40,000 Da and is a single polypeptide chain of about 180 amino acids. AGP is considered an acute phase reactant; its plasma levels increase several fold during acute infections. It belongs to the lipocalin family and binds numerous basic and neutral lipophilic drugs and steroid hormones. And this gene is located in 9q34.3-qter.

Aviva's human AGP1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for AGP1 has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: Q19-S201) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for AGP1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human AGP1 amount of sample captured in plate.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol