KLK11 ELISA Kit (Human)

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 217 1054 4898 232 994 4808 Standard deviation 12.8 83.26 240 16.93 92.44 259.63 CV (%) 5.9 7.9 4.9 7.3 9.3 5.4 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 217 1054 4898 232 994 4808 Mean (pg/ml) 217 1054 4898 232 994 4808 Standard deviation 12.8 83.26 240 16.93 92.44 259.63 Standard deviation 12.8 83.26 240 16.93 92.44 259.63 CV (%) 5.9 7.9 4.9 7.3 9.3 5.4 CV (%) 5.9 7.9 4.9 7.3 9.3 5.4

Hippostasin, hK11, Kallikrein 11, Kallikrein 11 isoform 1 preproprotein, Kallikrein 11 isoform 2 precursor, Kallikrein 11 precursor, Kallikrein related peptidase 11, Kallikrein-11 inactive chain 2, KLK 11, Klk11, KLK11_HUMAN, MGC33060, Protease serine 20 trypsin like, Protease serine trypsin like, PRSS20, Serine protease 20, TLSP, Trypsin like protease, Trypsin-like protease

Kallikrein-11 is a protein that in humans is encoded by the KLK11 gene. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in two transcript variants encoding two different isoforms which are differentially expressed.

Aviva's human KLK11 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for KLK11 has been precoated onto 96-well plates. Standards (NS0, E19-N250) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KLK11 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human KLK11 amount of sample captured in plate.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol