ADAMTS13 ELISA Kit (Human)

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1395 7236 20816 1322 6617 20301 Standard deviation 104.62 289.44 1165.69 104.43 291.14 1197.75 CV (%) 7.5 4 5.6 7.9 4.4 5.9 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1395 7236 20816 1322 6617 20301 Mean (pg/ml) 1395 7236 20816 1322 6617 20301 Standard deviation 104.62 289.44 1165.69 104.43 291.14 1197.75 Standard deviation 104.62 289.44 1165.69 104.43 291.14 1197.75 CV (%) 7.5 4 5.6 7.9 4.4 5.9 CV (%) 7.5 4 5.6 7.9 4.4 5.9

A disintegrin and metalloproteinase with thrombospondin motifs 13, A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 13, A disintegrin like and metalloprotease with thrombospondin type 1 motif 13, A disintegrin-like and metalloprotease (Reprolysin type) with thrombospondin type 1 motif 13, ADAM metallopeptidase with thrombospondin type 1 motif 13, ADAM TS, ADAM-TS 13, ADAM-TS13, ADAMTS 13, ADAMTS-13, ADAMTS13, ADAMTS13 protein, ATS13_HUMAN, C9orf8, EC 3.4.24.-, TTP, Von Willebrand factor cleaving protease, Von Willebrand factor cleaving protease, von Willebrand factor-cleaving protease, vWF cleaving protease, vWF CP, vWF-cleaving protease, vWF-CP, vWFCP

ADAMTS13 is a zinc-containing metalloprotease enzyme. The ADAMTS13 gene is mapped to chromosome 9q34 by genomic sequence analysis. It is reported that a metal-containing proteolytic enzyme (metalloprotease) in normal plasma cleaves the peptide bond between tyrosine at position 842 and methionine at position 843 in monomeric subunits of von Willebrand factor, thereby degrading the large multimers. And it is confirmed that the ADAMTS13 gene encodes the von Willebrand factor-cleaving protease (VWFCP) . ADAMTS13 is secreted in blood and degrades large vWf multimers, decreasing their activity.

Aviva's human ADAMTS13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for ADAMTS13 has been precoated onto 96-well plates. Standards (CHO, Q34-W688) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAMTS13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human ADAMTS13 amount of sample captured in plate.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol