What is the catalog number for TFPI ELISA Kit (Human)?

The catalog number for TFPI ELISA Kit (Human) is OKBB00829-96W.

What sizes are available for TFPI ELISA Kit (Human)?

TFPI ELISA Kit (Human) is available in the following size(s): 96 Well With Removable Strips.

Is TFPI ELISA Kit (Human) in stock?

TFPI ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

What happens if this product doesn’t perform as expected in my experiment?

Send us the catalogue number, product name, lot number, and a summary of your results. Our technical support team will investigate and, where needed, involve the manufacturer. This is covered under our guarantee provided the product was used before its expiry date and stored correctly.

TFPI ELISA Kit (Human)

Product Specifications

Reproducibility

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 57 238 848 55 237 897 Standard deviation 3.3 11.9 66.14 3.46 14.93 75.34 CV (%) 5.8 5 7.8 6.3 6.3 8.4 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 57 238 848 55 237 897 Mean (pg/ml) 57 238 848 55 237 897 Standard deviation 3.3 11.9 66.14 3.46 14.93 75.34 Standard deviation 3.3 11.9 66.14 3.46 14.93 75.34 CV (%) 5.8 5 7.8 6.3 6.3 8.4 CV (%) 5.8 5 7.8 6.3 6.3 8.4

CAS Number

7732-18-5

Gene Name

Tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)

Gene Aliases

EPI, Extrinsic pathway inhibitor, LACI, Lipoprotein-associated coagulation inhibitor, TFPI, TFPI1_HUMAN, Tissue factor pathway inhibitor

Gene ID

7035

Accession Number

NP_001027452.1

Reactivity

Human

Immunogen

Expression system for standard: NS0; Immunogen sequence: D29-K282

Target

TFPI (Tissue factor pathway inhibitor), also known as EPI, LACI or TFPI1, is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa) . The gene for TFPI is located on chromosome 2q31-q32.1, and has nine exons which span 70 kb. TFPI consists of a highly negatively-charged amino-terminus, three tandemly-linked Kunitz domains, and a highly positively-charged carboxy-terminus.TFPI contributes significantly to the inhibition of Xa in vivo, despite being present at concentrations of only 2.5 nM.

Type

ELISA Kit

Applications

ELISA

Assay Principle

Aviva's human TFPI ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for TFPI has been precoated onto 96-well plates. Standards (NS0, D29-K282) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TFPI is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human TFPI amount of sample captured in plate.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol