What is the catalog number for CA1 ELISA Kit (Human)?

The catalog number for CA1 ELISA Kit (Human) is OKBB00775-96W.

What sizes are available for CA1 ELISA Kit (Human)?

CA1 ELISA Kit (Human) is available in the following size(s): 96 Well With Removable Strips.

Is CA1 ELISA Kit (Human) in stock?

CA1 ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

Is this antibody conjugated?

Conjugation status, such as unconjugated, HRP, FITC, or biotin, is specified in the product name and confirmed on the datasheet.

What is the sensitivity of this ELISA kit?

Assay sensitivity, including the minimum detectable dose, is listed on the datasheet. Kits that are assembled on order typically ship within 2-4 weeks.

Do you ship this product with dry ice?

Some products, including certain cell lines and biologics, require dry ice for transportation, which carries an additional fee shown clearly in your quotation. Dry ice delivery isn’t always possible to every destination due to transit time or local restrictions, so we check feasibility in advance and you must store the goods at the correct temperature the moment they arrive.

CA1 ELISA Kit (Human)

Product Specifications

Reproducibility

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 452 2590 9786 584 2656 9986 Standard deviation 18.98 157.99 636.09 31.54 114.21 559.22 CV (%) 4.2 6.1 6.5 5.4 4.3 5.6 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 452 2590 9786 584 2656 9986 Mean (pg/ml) 452 2590 9786 584 2656 9986 Standard deviation 18.98 157.99 636.09 31.54 114.21 559.22 Standard deviation 18.98 157.99 636.09 31.54 114.21 559.22 CV (%) 4.2 6.1 6.5 5.4 4.3 5.6 CV (%) 4.2 6.1 6.5 5.4 4.3 5.6

CAS Number

7732-18-5

Gene Name

Carbonic anhydrase 1

Gene Aliases

Carbonic anhydrase 1; Carbonate dehydratase I; Carbonic anhydrase B; CAB; Carbonic anhydrase I; CA-I; CA1

Gene ID

759

Accession Number

NP_001122301.1

Reactivity

Human

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A2-F261

Target

Carbonic anhydrase 1 is an enzyme that in humans is encoded by the CA1 gene. It is a member of the Carbonic anhydrase. The CA1 gene is mapped to 8q22. CAI has got about 260 amino acids. This protein is highly expressed in erythrocytes. As catalysts of the reversible hydration of carbon dioxide, CAI participates in a variety of biologic processes like respiration, calcification, acid-base balance etc.

Type

ELISA Kit

Applications

ELISA

Assay Principle

Aviva's human CA1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CA1 has been precoated onto 96-well plates. Standards (Expression system for standard: E.coli, Immunogen sequence: A2-F261) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CA1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Fibronectin amount of sample captured in plate.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol