PRL ELISA Kit (Human)

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 278 1989 4706 274 1928 5020 Standard deviation 18.07 95.47 338.83 21.64 129.17 371.48 CV (%) 6.5 4.8 7.2 7.9 6.7 7.4 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 278 1989 4706 274 1928 5020 Mean (pg/ml) 278 1989 4706 274 1928 5020 Standard deviation 18.07 95.47 338.83 21.64 129.17 371.48 Standard deviation 18.07 95.47 338.83 21.64 129.17 371.48 CV (%) 6.5 4.8 7.2 7.9 6.7 7.4 CV (%) 6.5 4.8 7.2 7.9 6.7 7.4

AV290867, Lactogenic Hormone, Luteotropic Hormone, Mammotropin, OTTMUSP00000000651, PRL, Prl1a1, Prol, Prolactin precursor, RATPRLSD1, RNPROL, RP23-351I6.2, RP3-404K8.1

Prolactin, also calle PRL, is a protein that in humans is encodes by the PRL gene. This gene is mapped to 6p22.3. This gene encodes the anterior pituitary hormone prolactin. It may play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Prolactin also acts in a cytokine-like manner and as an important regulator of the immune system. Prolactin has important cell cycle related functions as a growth-, differentiating- and anti-apoptotic factor. As a growth factor binding to cytokine like receptors it has also profound influence on hematopoiesis, angiogenesis and is involved in the regulation of blood clotting through several pathways.

Aviva's human Prolactin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Prolactin has been precoated onto 96-well plates. Standards (E.coli, L29-C227) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Prolactin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Prolactin amount of sample captured in plate.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol