CCL19 ELISA Kit (Human)

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 29 234 421 27 213 398 Standard deviation 1.39 15.67 26.94 1.35 15.97 32.23 CV (%) 4.8 6.7 6.4 5 7.5 8.1 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 29 234 421 27 213 398 Mean (pg/ml) 29 234 421 27 213 398 Standard deviation 1.39 15.67 26.94 1.35 15.97 32.23 Standard deviation 1.39 15.67 26.94 1.35 15.97 32.23 CV (%) 4.8 6.7 6.4 5 7.5 8.1 CV (%) 4.8 6.7 6.4 5 7.5 8.1

Beta chemokine exodus 3, Beta-chemokine exodus-3, C C chemokine ligand 19, C-C motif chemokine 19, CC chemokine ligand 19, CCL19, CCL19_HUMAN, Chemokine (C C motif) ligand 19, Chemokine (CC motif) ligand 19, Chemokine CC Motif Ligand 19, CK beta 11, CK beta-11, CKb11, EBI1 ligand chemokine, ELC, Epstein-Barr virus-induced molecule 1 ligand chemokine, Exodus 3, Macrophage inflammatory protein 3 beta, MGC34433, MIP 3 beta, MIP-3-beta, MIP-3B, MIP3 beta, MIP3B, OTTHUMP00000000531, SCYA19, Small inducible cytokine A19, Small-inducible cytokine A19

Chemokine (C-C motif) ligand 19 (CCL19) is a small cytokine belonging to the CC chemokine family that is also known as EBI1 ligand chemokine (ELC) and macrophage inflammatory protein-3-beta (MIP-3-beta) . Using PCR of human-rodent hybrids, the CCL19 gene was map to human chromosome 9p13, and the CCL19 gene is not part of the CC chemokine gene cluster on chromosome 17. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs.

Aviva's human CCL19 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL19 has been precoated onto 96-well plates. Standards (E.coli, G22-S98) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL19 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human CCL19 amount of sample captured in plate.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol