What is the catalog number for FGF2 ELISA Kit (Human)?

The catalog number for FGF2 ELISA Kit (Human) is OKBB00699-96W.

What sizes are available for FGF2 ELISA Kit (Human)?

FGF2 ELISA Kit (Human) is available in the following size(s): 96 Well With Removable Strips.

Is FGF2 ELISA Kit (Human) in stock?

FGF2 ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

Does this product need any special customs documentation to be imported to my country?

If you’re outside the EU, UK, or USA, you’re responsible for customs clearance and any related taxes or fees. We provide the standard import documents, such as the Commercial Invoice, Packing List, and Airway Bill, in advance, so let us know early if your customs office needs anything additional for this specific product.

What buffer is this product supplied in?

The formulation or storage buffer is listed on the product datasheet. If you require a carrier-free or custom formulation, contact us before ordering, as this may involve a made-to-order production timeline.

What dilution range is recommended for immunohistochemistry (IHC) with this antibody?

A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.

FGF2 ELISA Kit (Human)

Product Specifications

Reproducibility

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 150 752 1076 135 781 1071 Standard deviation 10.95 54.89 79.62 10.8 62.48 92.1 CV (%) 7.3 7.3 7.4 8 8 8.6 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 150 752 1076 135 781 1071 Mean (pg/ml) 150 752 1076 135 781 1071 Standard deviation 10.95 54.89 79.62 10.8 62.48 92.1 Standard deviation 10.95 54.89 79.62 10.8 62.48 92.1 CV (%) 7.3 7.3 7.4 8 8 8.6 CV (%) 7.3 7.3 7.4 8 8 8.6

CAS Number

7732-18-5

Gene Name

Fibroblast growth factor 2

Gene Aliases

Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2; FGF2; FGFB

Gene ID

2247

Accession Number

NP_001997.5

Reactivity

Human

Immunogen

Expression system for standard: E.coli; Immunogen sequence:P143-S288

Target

FGF2 has been implicated in a multitude of physiologic and pathologic processes, including limb development, angiogenesis, wound healing, and tumor growth. Human FGF2 shares 96% and 97% amino acid sequence homology with mouse and rat respectively. FGF2 belongs to the fibroblast growth factor (FGF) family. Fibroblast growth factors (FGFs) exhibit widespread mitogenic and neurotrophic activities. Nine members of the family are currently known, and FGF-1 and FGF-2 are present in relatively high levels in CNS. FGF-2 is expressed by at low levels in many tissues and cell types and reaches high concentrations in brain and pituitary.

Type

ELISA Kit

Applications

ELISA

Assay Principle

Aviva's human FGF2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FGF2 has been precoated onto 96-well plates. Standards (E.coli, P143-S288) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human FGF2 amount of sample captured in plate.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol