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Fractalkine ELISA Kit (Rat)

Product Specifications

Reproducibility

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/mL) 1.54 7.5 14.3 1.32 7.7 15.4 SD 0.08 0.368 0.615 0.099 0.57 1.047 CV% 5.2 4.9 4.3 7.5 7.4 6.8 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/mL) 1.54 7.5 14.3 1.32 7.7 15.4 Mean (pg/mL) 1.54 7.5 14.3 1.32 7.7 15.4 SD 0.08 0.368 0.615 0.099 0.57 1.047 SD 0.08 0.368 0.615 0.099 0.57 1.047 CV% 5.2 4.9 4.3 7.5 7.4 6.8 CV% 5.2 4.9 4.3 7.5 7.4 6.8

CAS Number

7732-18-5

Gene Name

Chemokine (C-X3-C motif) ligand 1

Gene Aliases

A 152E5.2, AB030188, ABCD 3, ABCD3, C X3 C motif chemokine 1, C3Xkine, Chemokine (C X3 C motif) ligand 1, Chemokine C X3 C motif ligand 1, Chemokine CX3C Motif Ligand 1, CX3C membrane anchored chemokine, CXC3, CXC3C, FKN, Fractalkine, Neurotactin, NTN, NTT, SCYD 1, SCYD1, Small inducible cytokine D1, Small inducible cytokine subfamily D (Cys X3 Cys), member 1, Small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin), Small inducible cytokine subfamily D member 1

Gene ID

89808

Accession Number

NP_604450.1

Reactivity

Rat

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q25-T334

Type

ELISA Kit

Applications

ELISA

Assay Principle

Aviva's rat Fractalkine ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Fractalkine has been precoated onto 96-well plates. Standards (NS0, Q25-T334) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Fractalkine is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the rat Fractalkine amount of sample captured in plate.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA), and tissue homogenates

Detection Range

312 pg/ml - 20,000 pg/ml

Sensitivity

<10 pg/ml

Reconstitution

Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Specificity

Natural and recombinant rat Fractalkine

NCBI Gene Symbol

CX3CL1

Protein Name

Fractalkine

Gene Name URL

CX3CL1

Nucleotide Accession Number

NM_134455.1

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