Fractalkine ELISA Kit (Rat)
Product Specifications
Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/mL) 1.54 7.5 14.3 1.32 7.7 15.4 SD 0.08 0.368 0.615 0.099 0.57 1.047 CV% 5.2 4.9 4.3 7.5 7.4 6.8 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/mL) 1.54 7.5 14.3 1.32 7.7 15.4 Mean (pg/mL) 1.54 7.5 14.3 1.32 7.7 15.4 SD 0.08 0.368 0.615 0.099 0.57 1.047 SD 0.08 0.368 0.615 0.099 0.57 1.047 CV% 5.2 4.9 4.3 7.5 7.4 6.8 CV% 5.2 4.9 4.3 7.5 7.4 6.8
CAS Number
7732-18-5
Gene Name
Chemokine (C-X3-C motif) ligand 1
Gene Aliases
A 152E5.2, AB030188, ABCD 3, ABCD3, C X3 C motif chemokine 1, C3Xkine, Chemokine (C X3 C motif) ligand 1, Chemokine C X3 C motif ligand 1, Chemokine CX3C Motif Ligand 1, CX3C membrane anchored chemokine, CXC3, CXC3C, FKN, Fractalkine, Neurotactin, NTN, NTT, SCYD 1, SCYD1, Small inducible cytokine D1, Small inducible cytokine subfamily D (Cys X3 Cys), member 1, Small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin), Small inducible cytokine subfamily D member 1
Gene ID
89808
Accession Number
NP_604450.1
Reactivity
Rat
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q25-T334
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's rat Fractalkine ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Fractalkine has been precoated onto 96-well plates. Standards (NS0, Q25-T334) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Fractalkine is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the rat Fractalkine amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Cell culture supernatants, serum, plasma (heparin, EDTA), and tissue homogenates
Detection Range
312 pg/ml - 20,000 pg/ml
Sensitivity
<10 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant rat Fractalkine
NCBI Gene Symbol
CX3CL1
Protein Name
Fractalkine
Gene Name URL
CX3CL1
Nucleotide Accession Number
NM_134455.1
Frequently Asked Questions
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