Neuregulin-1/NRG1-beta1 ELISA Kit (Human)

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/mL) 675 1308 2147 694 1382 2503 SD 28.35 61.48 113.8 40.25 84.3 172.7 CV% 4.2 4.7 5.3 5.8 6.1 6.9 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/mL) 675 1308 2147 694 1382 2503 Mean (pg/mL) 675 1308 2147 694 1382 2503 SD 28.35 61.48 113.8 40.25 84.3 172.7 SD 28.35 61.48 113.8 40.25 84.3 172.7 CV% 4.2 4.7 5.3 5.8 6.1 6.9 CV% 4.2 4.7 5.3 5.8 6.1 6.9

Acetylcholine receptor inducing activity, Acetylcholine receptor-inducing activity, ARIA, Breast cancer cell differentiation factor p45, GGF, GGF2, Glial growth factor, Heregulin, HGL, HRG, HRG1, HRGA, MST131, NDF, Neu differentiation factor, Neuregulin 1, Neuregulin-1, Neuregulin1, nrg1, NRG1 beta 1, NRG1_HUMAN, Pro neuregulin 1, Pro NRG1, Pro-NRG1, Sensory and motor neuron derived factor, Sensory and motor neuron-derived factor, SMDF

Neuregulin 1 or NRG1 is a protein that in humans is encoded by the NRG1 gene. It is mapped to 8p12. NRG1 is one of four proteins in the neuregulin family that act on the EGFR family of receptors. The protein encoded by this gene is a membrane glycoprotein that mediates cell-cell signaling and plays a critical role in the growth and development of multiple organ systems. Dysregulation of this gene has been linked to diseases such as cancer, schizophrenia, and bipolar disorder (BPD) . It is also essential for the normal development of the nervous system and the heart. Furthermore, it was originally identified as a 44-kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues.

Aviva's human Neuregulin-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Neuregulin-1 has been precoated onto 96-well plates. Standards (E.coli, S2-K246) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Neuregulin-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Neuregulin-1 amount of sample captured in plate.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol