CXCL3 ELISA Kit (Rat)

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/mL) 170 375 602 161 352 593 SD 13.26 19.5 27.09 13.36 22.53 27.87 CV% 7.8 5.2 4.5 8.3 6.4 4.7 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/mL) 170 375 602 161 352 593 Mean (pg/mL) 170 375 602 161 352 593 SD 13.26 19.5 27.09 13.36 22.53 27.87 SD 13.26 19.5 27.09 13.36 22.53 27.87 CV% 7.8 5.2 4.5 8.3 6.4 4.7 CV% 7.8 5.2 4.5 8.3 6.4 4.7

C-X-C motif chemokine 3, Chemokine (CXC motif) ligand 3, Cinc 2, CINC 2b, Cinc2, CINC2b, CXCL 3, Cxcl3, CXCL3_HUMAN, Cytokine induced neutrophil chemoattractant 2, Dcip1, Dendritic cell inflammatory protein 1, Gm1960, GRO protein gamma, GRO-gamma, GRO-gamma (1-73), GRO-gamma (5-73), GRO3, GRO3 oncogene, GROG, Growth regulated protein gamma, Growth-regulated protein gamma, Macrophage inflammatory protein 2 beta precursor, Macrophage inflammatory protein 2-beta, Melanoma growth stimulatory activity gamma, Member 3, MGSA gamma, MIP 2b, MIP2-beta, MIP2B, SCYB3, Small inducible cytokine subfamily B

Chemokine (C-X-C motif) ligand 3 (CXCL3) is a small cytokine belonging to the CXC chemokine family. It is mapped to 14p2 in rat. CXCL3 controls migration and adhesion of monocytes and mediates its effects on its target cell by interacting with a cell surface chemokine receptor. It has been shown that CXCL3 regulates cell autonomously the migration of the precursors of cerebellar granule neurons toward the internal layers of cerebellum, during the morphogenesis of cerebellum. CXCL3 also play fundamental roles in the development, homeostasis and it has effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis.

Aviva's rat CXCL3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL3 has been precoated onto 96-well plates. Standards (E.coli, R33-S101) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the rat CXCL3 amount of sample captured in plate.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol