ADAMTS4 ELISA Kit (Human)

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/mL) 5.9 12 21 6.5 14 23 SD 0.248 0.612 1.2 0.377 0.882 1.66 CV% 4.2 5.1 5.7 5.8 6.3 7.2 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/mL) 5.9 12 21 6.5 14 23 Mean (pg/mL) 5.9 12 21 6.5 14 23 SD 0.248 0.612 1.2 0.377 0.882 1.66 SD 0.248 0.612 1.2 0.377 0.882 1.66 CV% 4.2 5.1 5.7 5.8 6.3 7.2 CV% 4.2 5.1 5.7 5.8 6.3 7.2

A disintegrin and metalloproteinase with thrombospondin motifs 4, A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 4, A disintegrin like and metalloprotease with thrombospondin type 1 motif 4, ADAM metallopeptidase with thrombospondin type 1 motif 4, ADAM TS 4, ADAM TS4, ADAM-TS 4, ADAM-TS4, ADAMTS 2, ADAMTS 4, ADAMTS 4, ADAMTS-4, ADAMTS2, ADAMTS4, ADAMTS4 protein, ADMP 1, ADMP-1, ADMP1, Aggrecanase 1, Aggrecanase 1, Aggrecanase-1, Aggrecanase1, ATS4_HUMAN, KIAA0688, KIAA0688

ADAMTS4, A disintegrin and metalloproteinase with thrombospondin motifs 4 is an enzyme that in humans is encoded by the ADAMTS4 gene. ADAMTS4 is a member of the large ADAMTS family of zinc-dependent proteases. The human ADAMTS4 gene is mapped to chromosome 1 by somatic cell hybrid analysis. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma.

Aviva's human ADAMTS4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ADAMTS4 has been precoated onto 96-well plates. Standards (CHO, F213-C685) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAMTS4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human ADAMTS4 amount of sample captured in plate.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol