CD70/TNFSF7/cd27L ELISA Kit (Mouse)

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/mL) 94 511 1032 148 525 1385 SD 3.57 26.06 44.38 6.66 32.55 77.56 CV% 3.8 5.1 4.3 4.5 6.2 5.6 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/mL) 94 511 1032 148 525 1385 Mean (pg/mL) 94 511 1032 148 525 1385 SD 3.57 26.06 44.38 6.66 32.55 77.56 SD 3.57 26.06 44.38 6.66 32.55 77.56 CV% 3.8 5.1 4.3 4.5 6.2 5.6 CV% 3.8 5.1 4.3 4.5 6.2 5.6

CD 27L, CD 70, CD27 L, CD27 LG, CD27 ligand, CD27-L, CD27L, CD27LG, CD70, CD70 antigen, CD70 molecule, CD70_HUMAN, Ki 24 antigen, Ki24 antigen, Surface antigen CD70, TNFSF 7, TNFSF7, Tumor necrosis factor (ligand) superfamily, member 7, Tumor necrosis factor ligand superfamily member

CD70 (Cluster of Differentiation 70), also known as TNFSF7 or CD27L, is a ligand for CD27. This gene is mapped to 19p13.3. CD70 is a surface antigen found on activated, but not resting, T and B lymphocytes. It has been found that CD70 induced proliferation of costimulated T cells and enhanced generation of cytolytic T cells. CD70 is homologous to the ligands of the TNF receptor family, including TNF-alpha, TNF-beta, and the CD40 ligand, showing 19 to 24% amino acid sequence identity in the extracellular region.

Aviva's mouse CD70 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD70 has been precoated onto 96-well plates. Standards (NS0, S45-P195) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD70 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse CD70 amount of sample captured in plate.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol