What is the catalog number for ANGPTL4 ELISA Kit (Human)?

The catalog number for ANGPTL4 ELISA Kit (Human) is OKBB00477-96W.

What sizes are available for ANGPTL4 ELISA Kit (Human)?

ANGPTL4 ELISA Kit (Human) is available in the following size(s): 96 Well With Removable Strips.

Is ANGPTL4 ELISA Kit (Human) in stock?

ANGPTL4 ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

How should this product be stored?

Storage conditions depend on the specific reagent and are listed on the product datasheet, with most antibodies and proteins requiring -20°C unless stated otherwise. It is your responsibility to store the product correctly immediately upon receipt; damage from improper storage after delivery is not eligible for replacement.

Is this product animal-origin-free?

Animal-origin-free status, where applicable, is noted on the datasheet under formulation details.

Does this product need any special customs documentation to be imported to my country?

If you’re outside the EU, UK, or USA, you’re responsible for customs clearance and any related taxes or fees. We provide the standard import documents, such as the Commercial Invoice, Packing List, and Airway Bill, in advance, so let us know early if your customs office needs anything additional for this specific product.

ANGPTL4 ELISA Kit (Human)

Product Specifications

Reproducibility

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/mL) 6.1 12.7 25.3 6.7 13.8 27.5 SD 0.35 0.75 1.54 0.52 1.12 2.28 CV% 5.7 5.9 6.1 7.8 8.1 8.3 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/mL) 6.1 12.7 25.3 6.7 13.8 27.5 Mean (pg/mL) 6.1 12.7 25.3 6.7 13.8 27.5 SD 0.35 0.75 1.54 0.52 1.12 2.28 SD 0.35 0.75 1.54 0.52 1.12 2.28 CV% 5.7 5.9 6.1 7.8 8.1 8.3 CV% 5.7 5.9 6.1 7.8 8.1 8.3

CAS Number

7732-18-5

Gene Name

Angiopoietin-like 4

Gene Aliases

425O18 1, Angiopoietin like 4, Angiopoietin related protein 4, ANGPT L2, ANGPT L4, ANGPTL2, ANGPTL4, Fasting induced adipose factor, FIAF, Fibrinogen/angiopoietin related protein, Hepatic fibrinogen/angiopoietin related protein, HFARP, Major histocompatibility complex region NG27, NL2, Peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin related protein, PGAR, pp1158, PPARG angiopoietin related protein, PSEC0166, Secreted protein Bk89, Weakly similar to angiopoietin 1

Gene ID

51129

Accession Number

NP_001034756.1

Reactivity

Human

Immunogen

Expression system for standard: NS0; Immunogen sequence: L165-S406

Target

Angiopoietin-related protein 4 (Angptl4) is a protein that in humans is encoded by the ANGPTL4 gene. This gene is a member of the angiopoietin/angiopoietin-like gene family and encodes a glycosylated, secreted protein with a fibrinogen C-terminal domain. This gene is induced under hypoxic conditions in endothelial cells and is the target of peroxisome proliferation activators. By radiation hybrid analysis, Angptl4 gene is mapped to 19p13.3. ANGPTL4 contributed to tumor growth and protected cells from anoikis, a form of programmed cell death induced when contact-dependent cells detach from the surrounding tissue matrix.

Type

ELISA Kit

Applications

ELISA

Assay Principle

Aviva's human ANGPTL4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ANGPTL4 has been precoated onto 96-well plates. Standards (NS0, L165-S406) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ANGPTL4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human ANGPTL4 amount of sample captured in plate.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol