DKK1 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/mL) 228 845 1474 259 897 1532 SD 12.54 56.6 91.39 17.1 66.4 105.7 CV% 5.5 6.7 6.2 6.6 7.4 6.9 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/mL) 228 845 1474 259 897 1532 Mean (pg/mL) 228 845 1474 259 897 1532 SD 12.54 56.6 91.39 17.1 66.4 105.7 SD 12.54 56.6 91.39 17.1 66.4 105.7 CV% 5.5 6.7 6.2 6.6 7.4 6.9 CV% 5.5 6.7 6.2 6.6 7.4 6.9
CAS Number
7732-18-5
Gene Name
Dickkopf WNT signaling pathway inhibitor 1
Gene Aliases
Dickkopf 1, Dickkopf homolog 1, Dickkopf related protein 1, DKK 1, hDkk 1, hDkk1, mDkk1, SK
Gene ID
13380
Accession Number
NP_034181.2
Reactivity
Mouse
Immunogen
Expression system for standard: CHO; Immunogen sequence: S30-H272
Target
Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene, which was mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.
Type
ELISA Kit
Applications
ELISA
Assay Principle
Aviva's mouse DKK1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for DKK1 has been precoated onto 96-well plates. Standards (CHO, S30-H272) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DKK1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse DKK1 amount of sample captured in plate.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Cell culture supernatants, serum and plasma (heparin, EDTA)
Detection Range
46.9 pg/ml - 3,000 pg/ml
Sensitivity
<15 pg/ml
Reconstitution
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Specificity
Natural and recombinant mouse DKK1
NCBI Gene Symbol
DKK1
Protein Name
Dickkopf-related protein 1
Gene Name URL
DKK1
Nucleotide Accession Number
NM_010051.3
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