ErbB-2 ELISA Kit (Human)

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Mean (pg/mL) 348 1057 2334 361 1049 2645 St. Dev. 14.62 45.45 91.03 21.3 67.14 140.2 %CV 4.20 4.29 3.90 5.90 6.40 5.30 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 Mean (pg/mL) 348 1057 2334 361 1049 2645 Mean (pg/mL) 348 1057 2334 361 1049 2645 St. Dev. 14.62 45.45 91.03 21.3 67.14 140.2 St. Dev. 14.62 45.45 91.03 21.3 67.14 140.2 %CV 4.20 4.29 3.90 5.90 6.40 5.30 %CV 4.20 4.29 3.90 5.90 6.40 5.30

C erb B2, C erbB 2, CD340, CD340 antigen, Cerb B2/neu protein, CerbB2, Erb B2, erbb2, HER 2, HER 2/neu, HER2, Her2/neu, Herstatin, Metastatic lymph node gene 19 protein, MLN 19, MLN19, NEU, NEU Proto Oncogene, Neuro Glioblastoma Derived Oncogene Homolog, Neuroblastoma/glioblastoma derived oncogene homolog, NGL, p185 ErbB2, p185erbB2, Proto oncogene c ErbB 2, Proto oncogene Neu, Receptor protein tyrosine kinase ErbB2 precursor, Receptor tyrosine protein kinase erbB 2, TKR1, Tyrosine kinase type cell surface receptor HER2, v erb b2 erythroblastic leukemia viral oncogene homolog 2 neuro/glioblastoma derived oncogene homolog (avian), Verb b2 erythroblastic leukemia viral oncogene homolog 2 neuro/glioblastoma derived oncogene homolog

HER2/neu (also known as ErbB-2) stands for "Human Epidermal growth factor Receptor 2" and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2/neu has also been designated as CD340 (cluster of differentiation 340) and p185. It is encoded by the ERBB2 gene.HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. It is encoded within the genome by HER2/neu, a known proto-oncogene. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. However, ErbB receptors dimerise on ligand binding, and HER2 is the preferential dimerisation partner of other members of the ErbB family.1 The HER2 gene is a proto-oncogene located at the long arm of human chromosome 17 (17q21-q22) 2.

Aviva's human ErbB-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for ErbB-2 has been precoated onto 96-well plates. Standards (NS0, T23-T652) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ErbB-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human ErbB-2 amount of sample captured in plate.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol