Cathepsin D ELISA Kit (Human)

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Mean (ng/mL) 1.24 3.37 6.73 1.38 3.51 6.82 St. Dev. 0.035 0.108 0.236 0.047 0.13 0.28 %CV 2.82 3.20 3.50 3.40 3.70 4.10 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 Mean (ng/mL) 1.24 3.37 6.73 1.38 3.51 6.82 Mean (ng/mL) 1.24 3.37 6.73 1.38 3.51 6.82 St. Dev. 0.035 0.108 0.236 0.047 0.13 0.28 St. Dev. 0.035 0.108 0.236 0.047 0.13 0.28 %CV 2.82 3.20 3.50 3.40 3.70 4.10 %CV 2.82 3.20 3.50 3.40 3.70 4.10

Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.

Aviva's human Cathepsin D ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Cathepsin D has been precoated onto 96-well plates. Standards (NS0, L21-L412) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cathepsin D is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Cathepsin D amount of sample captured in plate.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol