TPO ELISA Kit (Mouse)

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Mean (pg/mL) 296 611 1265 321 746 1464 St. Dev. 11.84 33 82.23 16.7 47 105.4 %CV 4 5.40 6.50 5.20 6.30 7.19 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 Mean (pg/mL) 296 611 1265 321 746 1464 Mean (pg/mL) 296 611 1265 321 746 1464 St. Dev. 11.84 33 82.23 16.7 47 105.4 St. Dev. 11.84 33 82.23 16.7 47 105.4 %CV 4 5.40 6.50 5.20 6.30 7.19 %CV 4 5.40 6.50 5.20 6.30 7.19

C mpl ligand, Megakaryocyte colony stimulating factor, Megakaryocyte growth and development factor, Megakaryocyte stimulating factor, MGC163194, MGDF, MKCSF, ML, MPL ligand, MPLLG, Myeloproliferative leukemia virus oncogene ligand, OTTHUMP00000210504, THPO, TPO

Thrombopoietin (TPO) is also called megakaryocyte growth and development factor (MGDF) . TPO, a lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from committed progenitor cells, is believed to be the major physiological regulator of circulating platelet levels.1 Human, dog, and mouse cDNAs for MGDF are highly conserved and encode open reading frames for proteins of 353, 352, and 356 amino acids, respectively, including predicted signal peptides. MGDF is a novel cytokine that regulates megakaryocyte development and is a ligand for the MPL receptor. The human MGDF gene has been mapped to chromosome 3q26.3. 2,3 The standard product used in this kit is gene recombinant mouse TPO, consisting of 335 amino acids with the molecular mass of 35KDa. As a result of glycosylation, the molecular mass is 75KDa.

Aviva's mouse TPO ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TPO has been precoated onto 96-well plates. Standards (NS0, S22-T356) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TPO is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse TPO amount of sample captured in plate.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol