IL1A ELISA Kit (Human)

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Mean (pg/mL) 54 147 221 48 139 234 St. Dev. 1.84 7.497 9.28 2.64 10.29 14.98 %CV 3.40 5.1 4.19 5.5 7.40 6.40 Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 Mean (pg/mL) 54 147 221 48 139 234 Mean (pg/mL) 54 147 221 48 139 234 St. Dev. 1.84 7.497 9.28 2.64 10.29 14.98 St. Dev. 1.84 7.497 9.28 2.64 10.29 14.98 %CV 3.40 5.1 4.19 5.5 7.40 6.40 %CV 3.40 5.1 4.19 5.5 7.40 6.40

Hematopoietin 1, IL 1 alpha, Il 1a, IL1, IL1 ALPHA, IL1A, IL1F1, Ilia, Interleukin 1 alpha, Interleukin 1 alpha precursor, Interleukin1 alpha, OTTHUMP00000203692, OTTMUSP00000016430, Preinterleukin 1, Pro interleukin 1, Pro interleukin 1 alpha, Prointerleukin 1, RP23-160G19.8

Interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta (IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair.1 IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases.2 Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1 alpha gene is assigned to chromosome 2.3 Genetic polymorphisms at interleukin (IL) -1alpha and IL-1beta have been recently suggested to be associated with severity of adult periodontitis.4 The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis.5 The standard product used in this kit is recombinant human IL-1α with the molecular mass of 18kDa.

Aviva's human IL-1 alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-1 alpha has been precoated onto 96-well plates. Standards (E.coli, S113-A271) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1 alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human IL-1 alpha amount of sample captured in plate.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol