Aldosterone ELISA Kit

Sample Aldosterone Conc. (pg/mL) %CV 1 1,018.7 6.0 2 156.2 5.9 3 40.6 8.8 Sample Aldosterone Conc. (pg/mL) %CV Sample Aldosterone Conc. (pg/mL) %CV 1 1,018.7 6.0 1 1,018.7 6.0 2 156.2 5.9 2 156.2 5.9 3 40.6 8.8 3 40.6 8.8 Sample Aldosterone Conc. (pg/mL) %CV 1 1,051.9 20.5 2 150.2 12.2 3 39.6 25.8 Sample Aldosterone Conc. (pg/mL) %CV Sample Aldosterone Conc. (pg/mL) %CV 1 1,051.9 20.5 1 1,051.9 20.5 2 150.2 12.2 2 150.2 12.2 3 39.6 25.8 3 39.6 25.8

The DetectX® Aldosterone Immunoassay kit is designed to quantitatively measure Aldosterone present in extracted serum and plasma, or in saliva, urine, extracted dried fecal samples, and tissue culture media samples. Please read the complete kit insert before performing this assay. This kit measures total aldosterone in extracted serum or plasma and fecal samples. An aldosterone stock solution is provided to generate a standard curve for the assay and all samples should be read off the standard curve. We provide protocols on pages 8 and 12 to prepare assay standards from 4,000 to 3.906 pg/mL or from 5,000 to 8.192 pg/mL. Please choose the standard range that fits your sample concentrations most appropriately. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies. An aldosterone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to aldosterone to each well. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound aldosteroneperoxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the aldosterone in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Component Quantity Coated Clear 96 Well Plate 1 or 5 Each Aldosterone Standard 125 uL or 625 uL DetectX® Aldosterone Antibody 3 mL or 13 mL DetectX® Aldosterone Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 Each Component Quantity Component Quantity Coated Clear 96 Well Plate 1 or 5 Each Coated Clear 96 Well Plate 1 or 5 Each Aldosterone Standard 125 uL or 625 uL Aldosterone Standard 125 uL or 625 uL DetectX® Aldosterone Antibody 3 mL or 13 mL DetectX® Aldosterone Antibody 3 mL or 13 mL DetectX® Aldosterone Conjugate 3 mL or 13 mL DetectX® Aldosterone Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Assay Buffer Concentrate 28 mL or 55 mL Wash Buffer Concentrate 30 mL or 125 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 Each Plate Sealer 1 or 5 Each