Catalase Colorimetric Activity Kit

Sample Catalase Activity (U/mL) %CV 1 1.71 3.5 2 0.84 4.0 3 0.48 4.8 Sample Catalase Activity (U/mL) %CV Sample Catalase Activity (U/mL) %CV 1 1.71 3.5 1 1.71 3.5 2 0.84 4.0 2 0.84 4.0 3 0.48 4.8 3 0.48 4.8 Sample Catalase Activity (U/mL) %CV 1 1.79 11.9 2 0.94 9.8 3 0.53 12.3 Sample Catalase Activity (U/mL) %CV Sample Catalase Activity (U/mL) %CV 1 1.79 11.9 1 1.79 11.9 2 0.94 9.8 2 0.94 9.8 3 0.53 12.3 3 0.53 12.3

The DetectX® Catalase Activity Kit is designed to quantitatively measure catalase activity in a variety of samples. Please read the complete kit insert before performing this assay. A bovine catalase standard is provided to generate a standard curve for the assay and all samples should be read off of the standard curve. Samples are diluted in the provided Assay Buffer and added to the wells of a half area clear plate. Hydrogen peroxide is added to each well and the plate incubated at room temperature for 30 minutes. The supplied Substrate is added, followed by diluted horseradish peroxidase and incubated at room temperature for 15 minutes. The HRP reacts with the substrate in the presence of hydrogen peroxide to convert the colorless substrate into a pink-colored product. The colored product is read at 560 nm. Increasing levels of catalase in the samples causes a decrease in H2O2 concentration and a reduction in pink product. The activity of the catalase in the sample is calculated after making a suitable correction for any dilution, using software available with most plate readers. The results are expressed in terms of units of catalase activity per mL.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Component Quantity Clear Half Area 96 Well Plates 2 Plates Catalase Standard 90 uL Assay Buffer Concentrate 25 mL Hydrogen Peroxide Reagent 5 mL Substrate 5 mL Horseradish Peroxidase Concentrate 120 uL Component Quantity Component Quantity Clear Half Area 96 Well Plates 2 Plates Clear Half Area 96 Well Plates 2 Plates Catalase Standard 90 uL Catalase Standard 90 uL Assay Buffer Concentrate 25 mL Assay Buffer Concentrate 25 mL Hydrogen Peroxide Reagent 5 mL Hydrogen Peroxide Reagent 5 mL Substrate 5 mL Substrate 5 mL Horseradish Peroxidase Concentrate 120 uL Horseradish Peroxidase Concentrate 120 uL