Corticosterone ELISA Kit

Sample Corticosterone Conc. (pg/mL) %CV 1 2,460.6 6.3 2 601.5 6.5 3 371.6 3.1 4 259.0 4.8 Sample Corticosterone Conc. (pg/mL) %CV Sample Corticosterone Conc. (pg/mL) %CV 1 2,460.6 6.3 1 2,460.6 6.3 2 601.5 6.5 2 601.5 6.5 3 371.6 3.1 3 371.6 3.1 4 259.0 4.8 4 259.0 4.8 Sample Corticosterone Conc. (pg/mL) %CV 1 2,618.3 7.5 2 630.1 6.4 3 267.9 9.9 Sample Corticosterone Conc. (pg/mL) %CV Sample Corticosterone Conc. (pg/mL) %CV 1 2,618.3 7.5 1 2,618.3 7.5 2 630.1 6.4 2 630.1 6.4 3 267.9 9.9 3 267.9 9.9

The DetectX® Corticosterone Immunoassay kit is designed to quantitatively measure Corticosterone present in serum, plasma, saliva, urine, extracted dried fecal samples, and tissue culture media samples. Please read the complete kit insert before performing this assay. This kit measures total corticosterone in serum and plasma and in extracted fecal samples. The kit offers two standard curve ranges. Depending on the anticipated sensitivity needed for your samples, you can use 50 uL of standads and samples with an assay range of 10,000 pg/mL to 39.063 pg/mL or use 100 uL of standards and samples with an assay range of 5,000 to 19.53 pg/mL. A corticosterone stock solution is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Please choose the standard range that fits your sample concentrations most appropriately. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies. A corticosterone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to corticosterone to each well. After an hour incubation the plate is washed and substrate is added. The substrate reacts with the bound corticosterone-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450nm wavelength. The concentration of the corticosterone in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Component Quantity Coated Clear 96 Well Plates 1 or 5 Each Corticosterone Standard 125 uL or 625 uL DetectX® Corticosterone Antibody 3 mL or 13 mL DetectX® Corticosterone Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Dissociation Reagent 1 mL or 5 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 Each Component Quantity Component Quantity Coated Clear 96 Well Plates 1 or 5 Each Coated Clear 96 Well Plates 1 or 5 Each Corticosterone Standard 125 uL or 625 uL Corticosterone Standard 125 uL or 625 uL DetectX® Corticosterone Antibody 3 mL or 13 mL DetectX® Corticosterone Antibody 3 mL or 13 mL DetectX® Corticosterone Conjugate 3 mL or 13 mL DetectX® Corticosterone Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Assay Buffer Concentrate 28 mL or 55 mL Dissociation Reagent 1 mL or 5 mL Dissociation Reagent 1 mL or 5 mL Wash Buffer Concentrate 30 mL or 125 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 Each Plate Sealer 1 or 5 Each