Estradiol Serum ELISA Kit

Sample Estradiol Conc. (pg/mL) %CV 1 58.8 2.4 2 16.1 4.1 3 15.3 5.1 Sample Estradiol Conc. (pg/mL) %CV Sample Estradiol Conc. (pg/mL) %CV 1 58.8 2.4 1 58.8 2.4 2 16.1 4.1 2 16.1 4.1 3 15.3 5.1 3 15.3 5.1 Sample Estradiol Conc. (pg/mL) %CV 1 65.9 9.8 2 16.5 9.6 3 16.4 7.4 Sample Estradiol Conc. (pg/mL) %CV Sample Estradiol Conc. (pg/mL) %CV 1 65.9 9.8 1 65.9 9.8 2 16.5 9.6 2 16.5 9.6 3 16.4 7.4 3 16.4 7.4

The DetectX® Serum Estradiol Immunoassay kit uses a specifically generated antibody to measure estradiol in serum and plasma samples. Please read the complete kit insert before performing this assay. An estradiol standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies. An estradiol-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a sheep antibody to estradiol to each well. After a 2 hour incubation the plate is washed and substrate is added. The substrate reacts with the bound estradiol-peroxidase conjugate. After a short 30 minute incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the estradiol in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Component Quantity Coated Clear 96 Well Plate 1 or 5 Each Estradiol Standard 75 uL or 375 uL DetectX® Serum Estradiol Antibody 3 mL or 13 mL DetectX® Serum Estradiol Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 Each Component Quantity Component Quantity Coated Clear 96 Well Plate 1 or 5 Each Coated Clear 96 Well Plate 1 or 5 Each Estradiol Standard 75 uL or 375 uL Estradiol Standard 75 uL or 375 uL DetectX® Serum Estradiol Antibody 3 mL or 13 mL DetectX® Serum Estradiol Antibody 3 mL or 13 mL DetectX® Serum Estradiol Conjugate 3 mL or 13 mL DetectX® Serum Estradiol Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Assay Buffer Concentrate 28 mL or 55 mL Wash Buffer Concentrate 30 mL or 125 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 Each Plate Sealer 1 or 5 Each