ANP ELISA Kit

Sample ANP Conc. (ng/mL) %CV 1 27.2 3.3 2 11.3 4.8 3 5.33 8.3 Sample ANP Conc. (ng/mL) %CV Sample ANP Conc. (ng/mL) %CV 1 27.2 3.3 1 27.2 3.3 2 11.3 4.8 2 11.3 4.8 3 5.33 8.3 3 5.33 8.3 Sample ANP Conc. (ng/mL) %CV 1 26.4 8.7 2 10.9 9.2 3 4.9 8.3 Sample ANP Conc. (ng/mL) %CV Sample ANP Conc. (ng/mL) %CV 1 26.4 8.7 1 26.4 8.7 2 10.9 9.2 2 10.9 9.2 3 4.9 8.3 3 4.9 8.3

The DetectX® ANP Immunoassay Kit is designed to quantitatively measure ANP present in plasma, urine, and tissue culture media samples. An ANP standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An ANP-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of an antibody to ANP to each well. After an hour incubation the plate is washed and substrate is added. The substrate reacts with the bound ANP-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the ANP in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Component Quantity Coated Clear 96 Well Plates 1 or 5 Each Catalog Number X016-1EA ANP Standard 125 uL or 625 uL Catalog Number C095-125UL or -625UL DetectX® ANP Antibody 3 mL or 13 mL Catalog Number C262-3ML or -13ML DetectX® ANP Conjugate 3 mL or 13 mL Catalog Number C094-3ML or -13ML Assay Buffer Concentrate 28 mL or 55 mL Catalog Number X065-28ML or -55ML Extraction Solution 50 mL or 250 mL Catalog Number X123-50ML or -250ML Wash Buffer Concentrate 30 mL or 125 mL Catalog Number X007-30ML or -125ML TMB Substrate 11 mL or 55 mL Catalog Number X019-11ML or -55ML Stop Solution 5 mL or 25 mL Catalog Number X020-5ML or -25ML Plate Sealer 1 or 5 Each Component Quantity Component Quantity Coated Clear 96 Well Plates 1 or 5 Each Catalog Number X016-1EA Coated Clear 96 Well Plates 1 or 5 Each Catalog Number X016-1EA ANP Standard 125 uL or 625 uL Catalog Number C095-125UL or -625UL ANP Standard 125 uL or 625 uL Catalog Number C095-125UL or -625UL DetectX® ANP Antibody 3 mL or 13 mL Catalog Number C262-3ML or -13ML DetectX® ANP Antibody 3 mL or 13 mL Catalog Number C262-3ML or -13ML DetectX® ANP Conjugate 3 mL or 13 mL Catalog Number C094-3ML or -13ML DetectX® ANP Conjugate 3 mL or 13 mL Catalog Number C094-3ML or -13ML Assay Buffer Concentrate 28 mL or 55 mL Catalog Number X065-28ML or -55ML Assay Buffer Concentrate 28 mL or 55 mL Catalog Number X065-28ML or -55ML Extraction Solution 50 mL or 250 mL Catalog Number X123-50ML or -250ML Extraction Solution 50 mL or 250 mL Catalog Number X123-50ML or -250ML Wash Buffer Concentrate 30 mL or 125 mL Catalog Number X007-30ML or -125ML Wash Buffer Concentrate 30 mL or 125 mL Catalog Number X007-30ML or -125ML TMB Substrate 11 mL or 55 mL Catalog Number X019-11ML or -55ML TMB Substrate 11 mL or 55 mL Catalog Number X019-11ML or -55ML Stop Solution 5 mL or 25 mL Catalog Number X020-5ML or -25ML Stop Solution 5 mL or 25 mL Catalog Number X020-5ML or -25ML Plate Sealer 1 or 5 Each Plate Sealer 1 or 5 Each