Androstenedione ELISA Kit

Sample Androstenedione Conc. (pg/mL) %CV 1 226.2 6.6 2 116.0 9.4 3 57.1 13.9 4 31.7 9.2 Sample Androstenedione Conc. (pg/mL) %CV Sample Androstenedione Conc. (pg/mL) %CV 1 226.2 6.6 1 226.2 6.6 2 116.0 9.4 2 116.0 9.4 3 57.1 13.9 3 57.1 13.9 4 31.7 9.2 4 31.7 9.2 Sample Androstenedione Conc. (pg/mL) %CV 1 232.4 9.7 2 119.3 9.3 3 58.9 7.4 4 34.2 12.1 Sample Androstenedione Conc. (pg/mL) %CV Sample Androstenedione Conc. (pg/mL) %CV 1 232.4 9.7 1 232.4 9.7 2 119.3 9.3 2 119.3 9.3 3 58.9 7.4 3 58.9 7.4 4 34.2 12.1 4 34.2 12.1

The DetectX® Androstenedione Immunoassay Kit uses a specifically generated antibody to measure androstenedione in saliva or in extracted serum and plasma. This kit is not recommended for serum or plasma samples without extraction. The kit will also quantitatively measure androstenedione present in reconstituted buffer samples and tissue culture media samples. Please read the complete kit insert before performing this assay. An androstenedione standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies. An androstenedione-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to androstenedione to each well. After a two hour incubation the plate is washed and substrate added. The substrate reacts with the bound androstenedione-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. After making suitable correction for the dilution of the sample, the concentration of the androstenedione in the sample is calculated using software available with most plate readers.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Component Quantity Coated Clear 96 Well Plates 1 or 5 Each Androstenedione Standard 125 uL or 625 uL DetectX® Androstenedione Antibody 3 mL or 13 mL DetectX® Androstenedione Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 Each Component Quantity Component Quantity Coated Clear 96 Well Plates 1 or 5 Each Coated Clear 96 Well Plates 1 or 5 Each Androstenedione Standard 125 uL or 625 uL Androstenedione Standard 125 uL or 625 uL DetectX® Androstenedione Antibody 3 mL or 13 mL DetectX® Androstenedione Antibody 3 mL or 13 mL DetectX® Androstenedione Conjugate 3 mL or 13 mL DetectX® Androstenedione Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Assay Buffer Concentrate 28 mL or 55 mL Wash Buffer Concentrate 30 mL or 125 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 Each Plate Sealer 1 or 5 Each