CRP ELISA Kit

Sample C-Reactive Protein Conc. (ng/mL) %CV 1 40.65 7.8 2 31.17 8.0 3 19.37 7.0 Sample C-Reactive Protein Conc. (ng/mL) %CV Sample C-Reactive Protein Conc. (ng/mL) %CV 1 40.65 7.8 1 40.65 7.8 2 31.17 8.0 2 31.17 8.0 3 19.37 7.0 3 19.37 7.0 Sample C-Reactive Protein Conc. (ng/mL) %CV 1 41.09 9.9 2 29.72 11.5 3 16.92 9.7 Sample C-Reactive Protein Conc. (ng/mL) %CV Sample C-Reactive Protein Conc. (ng/mL) %CV 1 41.09 9.9 1 41.09 9.9 2 29.72 11.5 2 29.72 11.5 3 16.92 9.7 3 16.92 9.7

The human C-Reactive Protein (CRP) ELISA Kit is designed to quantitatively measure CRP present in a variety of samples. Please read the complete kit insert before performing this assay. A C-Reactive Protein standard, calibrated to the WHO 1st International Reference Preparation 85/506, is provided to generate a standard curve for the assay and all samples should be read off the standard curve. A peroxidase-conjugated CRP monoclonal antibody is pipetted into a clear microtiter plate coated with a monoclonal antibody to capture CRP present in the sample. Samples or standards are added and the plate is incubated for 2 hours and washed. Substrate is then added to the plate, which reacts with the bound CRP conjugated antibody. After incubation, the substrate reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of CRP in the sample is calculated, after making suitable correction for dilution, using software available with most plate readers.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Component Quantity Mouse anti-C-Reactive Protein Clear Coated 96 Well Plate 1 or 5 Each C-Reactive Protein Standard 40 uL or 200 uL C-Reactive Protein Conjugate 5 mL or 25 mL Assay Buffer Concentrate 28 mL or 80 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 each Component Quantity Component Quantity Mouse anti-C-Reactive Protein Clear Coated 96 Well Plate 1 or 5 Each Mouse anti-C-Reactive Protein Clear Coated 96 Well Plate 1 or 5 Each C-Reactive Protein Standard 40 uL or 200 uL C-Reactive Protein Standard 40 uL or 200 uL C-Reactive Protein Conjugate 5 mL or 25 mL C-Reactive Protein Conjugate 5 mL or 25 mL Assay Buffer Concentrate 28 mL or 80 mL Assay Buffer Concentrate 28 mL or 80 mL Wash Buffer Concentrate 30 mL or 125 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 each Plate Sealer 1 or 5 each