2'3'-Cylic GAMP ELISA Kit

_x005F Sample 2',3'-Cyclic GAMP Conc. (pmol/mL) %CV _x005F 1 1.08 6.2 _x005F 2 5.14 6.1 _x005F 3 10.4 5.7 _x005F Sample 2',3'-Cyclic GAMP Conc. (pmol/mL) %CV Sample 2',3'-Cyclic GAMP Conc. (pmol/mL) %CV 1 1.08 6.2 1 1.08 6.2 2 5.14 6.1 2 5.14 6.1 3 10.4 5.7 3 10.4 5.7 _x005F Sample 2',3'-Cyclic GAMP Conc. (pmol/mL) %CV _x005F 1 1.00 8.5 _x005F 2 4.92 6.7 _x005F 3 9.97 8.0 _x005F Sample 2',3'-Cyclic GAMP Conc. (pmol/mL) %CV Sample 2',3'-Cyclic GAMP Conc. (pmol/mL) %CV 1 1.00 8.5 1 1.00 8.5 2 4.92 6.7 2 4.92 6.7 3 9.97 8.0 3 9.97 8.0

The DetectX® Direct 2',3'-Cyclic GAMP (cGAMP) Immunoassay Kit is designed to quantitatively measure 2',3'-cGAMP present in lysed cells and tissue, EDTA plasma, and tissue culture media samples. Please read the complete kit insert before performing this assay. A 2',3'-cGAMP standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. A clear microtiter plate coated with an antibody to capture rabbit IgG is provided. Standards or diluted samples are pipetted into the wells. A 2',3'-cGAMP-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a rabbit polyclonal antibody to 2',3'-cGAMP to each well. After a 2 hour incubation, the plate is washed and substrate is added. The substrate reacts with the bound 2',3'-cGAMP-peroxidase conjugate and after a short incubation the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the 2',3'-cGAMP in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

_x005F Component Quantity _x005F Coated Clear 96 Well Plates 1 or 5 Each _x005F 2',3'-Cyclic GAMP Standard 125 uL or 625 uL _x005F DetectX® 2',3'-Cyclic GAMP Antibody 3 mL or 13 mL _x005F DetectX® 2',3-Cyclic GAMP Conjugate 3 mL or 13 mL _x005F Assay Buffer Concentrate 28 mL or 55 mL _x005F Wash Buffer Concentrate 30 mL or 125 mL _x005F TMB Substrate 11 mL or 55 mL _x005F Stop Solution 5 mL or 25 mL _x005F Plate Sealer 1 or 5 Each _x005F Component Quantity Component Quantity Coated Clear 96 Well Plates 1 or 5 Each Coated Clear 96 Well Plates 1 or 5 Each 2',3'-Cyclic GAMP Standard 125 uL or 625 uL 2',3'-Cyclic GAMP Standard 125 uL or 625 uL DetectX® 2',3'-Cyclic GAMP Antibody 3 mL or 13 mL DetectX® 2',3'-Cyclic GAMP Antibody 3 mL or 13 mL DetectX® 2',3-Cyclic GAMP Conjugate 3 mL or 13 mL DetectX® 2',3-Cyclic GAMP Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Assay Buffer Concentrate 28 mL or 55 mL Wash Buffer Concentrate 30 mL or 125 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 Each Plate Sealer 1 or 5 Each