RBP ELISA Kit

Sample RBP Conc. (ng/mL) %CV 1 52.6 6.1 2 120.8 4.8 3 182.6 3.7 Sample RBP Conc. (ng/mL) %CV Sample RBP Conc. (ng/mL) %CV 1 52.6 6.1 1 52.6 6.1 2 120.8 4.8 2 120.8 4.8 3 182.6 3.7 3 182.6 3.7 Sample RBP Conc. (ng/mL) %CV 1 45.3 16.0 2 112.0 11.6 3 174.6 9.3 Sample RBP Conc. (ng/mL) %CV Sample RBP Conc. (ng/mL) %CV 1 45.3 16.0 1 45.3 16.0 2 112.0 11.6 2 112.0 11.6 3 174.6 9.3 3 174.6 9.3 Sample RBP Conc. (ng/mL) %CV 1 8.2 11.4 2 19.9 9.3 3 31.5 9.9 Sample RBP Conc. (ng/mL) %CV Sample RBP Conc. (ng/mL) %CV 1 8.2 11.4 1 8.2 11.4 2 19.9 9.3 2 19.9 9.3 3 31.5 9.9 3 31.5 9.9

The DetectX® Multi-Format Retinol Binding Protein (RBP) kit is designed to quantitatively measure RBP present in serum, plasma, dried blood spot (DBS) and urine samples. Please read the complete kit insert before performing this assay. The kit offers two standard curve ranges. For serum and plasma samples, we recommend using 10 uL of standards and samples with an assay range of 1,000 to 7.813 ng/mL. For urine and dried blood spot samples, we recommend using 100 uL of standards and samples with an assay range of 200 to 1.563 ng/mL. A RBP standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies. A RBP-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of the RBP polyclonal antibody to each well. After an hour incubation the plate is washed and substrate is added. The substrate reacts with the bound RBP-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of RBP in the sample is calculated, after making a suitable correction for the dilution of the sample, using software available with most plate readers.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Component Quantity Coated Clear 96 Well Plates 1 or 5 Each RBP Standard 0 uL or 240 uL DetectX® RBP Antibody 3 mL or 13 mL DetectX® RBP Conjugate 3 mL or 13 mL Assay Buffer Concentrate 8 mL or 55 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 Each Component Quantity Component Quantity Coated Clear 96 Well Plates 1 or 5 Each Coated Clear 96 Well Plates 1 or 5 Each RBP Standard 0 uL or 240 uL RBP Standard 0 uL or 240 uL DetectX® RBP Antibody 3 mL or 13 mL DetectX® RBP Antibody 3 mL or 13 mL DetectX® RBP Conjugate 3 mL or 13 mL DetectX® RBP Conjugate 3 mL or 13 mL Assay Buffer Concentrate 8 mL or 55 mL Assay Buffer Concentrate 8 mL or 55 mL Wash Buffer Concentrate 30 mL or 125 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 Each Plate Sealer 1 or 5 Each