Allopregnanolone ELISA Kit

Sample Allopregnanolone Conc. (pg/mL) %CV 1 3,576.5 3.7 2 1,768.8 4.6 3 545.7 12.9 Sample Allopregnanolone Conc. (pg/mL) %CV Sample Allopregnanolone Conc. (pg/mL) %CV 1 3,576.5 3.7 1 3,576.5 3.7 2 1,768.8 4.6 2 1,768.8 4.6 3 545.7 12.9 3 545.7 12.9 Sample Allopregnanolone Conc. (pg/mL) %CV 1 3,538.8 6.2 2 1,710.6 7.0 3 499.2 11.2 Sample Allopregnanolone Conc. (pg/mL) %CV Sample Allopregnanolone Conc. (pg/mL) %CV 1 3,538.8 6.2 1 3,538.8 6.2 2 1,710.6 7.0 2 1,710.6 7.0 3 499.2 11.2 3 499.2 11.2

The DetectX® Allopregnanolone Immunoassay Kit is designed to quantitatively measure allopregnanolone present in extracted serum, plasma, or dried fecal samples, or in diluted urine and tissue culture media samples. Please read the complete kit insert before performing this assay. An allopregnanolone standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse antibodies. An allopregnanolone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a monoclonal antibody to allopregnanolone to each well. The kit has two format options: A primary incubation of 2 hours at room temperature with shaking, or overnight at 4C. At the end of the incubation period the plate is washed and substrate is added. The substrate reacts with the bound allopregnanolone-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the allopregnanolone in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Component Quantity Coated Clear 96 Well Plates 1 or 5 Each Allopregnanolone Standard 125 uL or 625 uL DetectX® Allopregnanolone Antibody 3 mL or 13 mL DetectX® Allopregnanolone Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 Each Component Quantity Component Quantity Coated Clear 96 Well Plates 1 or 5 Each Coated Clear 96 Well Plates 1 or 5 Each Allopregnanolone Standard 125 uL or 625 uL Allopregnanolone Standard 125 uL or 625 uL DetectX® Allopregnanolone Antibody 3 mL or 13 mL DetectX® Allopregnanolone Antibody 3 mL or 13 mL DetectX® Allopregnanolone Conjugate 3 mL or 13 mL DetectX® Allopregnanolone Conjugate 3 mL or 13 mL Assay Buffer Concentrate 28 mL or 55 mL Assay Buffer Concentrate 28 mL or 55 mL Wash Buffer Concentrate 30 mL or 125 mL Wash Buffer Concentrate 30 mL or 125 mL TMB Substrate 11 mL or 55 mL TMB Substrate 11 mL or 55 mL Stop Solution 5 mL or 25 mL Stop Solution 5 mL or 25 mL Plate Sealer 1 or 5 Each Plate Sealer 1 or 5 Each